Lab Report of the Experiment of Conjugation of E. Coli

Abstract:Conjugation is a natural devolvering process that involves the carry of deoxyribonucleic acid from one electric cellphone into another by means of a tangible connection between the cells. In the future(a) experiment, 2 gos of Esche productiveia coli bacteriuml cells ( giver F?lac+strs and liquidator F-lac-strr) underwent conjugation to produce a transconjugant strain (F?lac+strr). MAC exfoliations and streptomycin were utilized to determine if conjugation had occur expiration. When plated, the bestower colonies appeared red and the recipient colonies appeared white. The transconjugant plates showed red and white colonies. developing alkaline lysis miniprep, a DNA plasmid was isolated from the donor and transconjugant strains and FIGE electrophoresis was apply to determine the size of the plasmid. The conjugation cleverness was found to be 16.25% and the plasmid DNA was approximately 97 kilobases long. The results show that the F? plasmid was effectiv ely transferred from the donor cells into the recipient cells via conjugation. Introduction:bacterial conjugation is the unidirectional transfer of all genomic DNA or plasmid DNA from a donor bacterial cell to a recipient bacterial cell by cell-to-cell affect via a sex pilus (Snustad & international ampere; Simmons, 2006). Conjugation was for the first time discovered by Lederberg and Tatum in 1946. In their experiment, they grew two strains of bacteria in separate vessels with rich medium and then in concert in one vessel containing the same medium.

Then, they spread the ternion vessel table of contents onto m edium agar plates and incubated them overnig! ht at 37?C. The moreover plate that showed cell growth was the plate containing the mixture of the two bacterial strains. The other two plates showed no growth. This experiment proven that in order for recombination to occur, the two strains mustiness come in affair with one another (Lederberg, Tatum, 1946). In 1950, Bernard Davis discovered that cell-to-cell contact was required to obtain a transconjugant. Using a U tube containing a sintered... If you want to fit a integral essay, order it on our website: BestEssayCheap.com

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